Phosphate Buffer (PB Buffer) is widely used in biological and chemical research due to its ability to maintain a stable pH. It is commonly employed in cell culture applications to provide an optimal environment for cell growth and maintenance. In molecular biology, PB Buffer is essential for procedures such as DNA and RNA extraction, as well as protein purification, where pH stability is crucial. It is also utilized in immunohistochemistry and immunofluorescence techniques to preserve tissue structure and antigen integrity during staining processes. Additionally, PB Buffer is a key component in various diagnostic assays, including ELISA, where it ensures accurate and reproducible results. Its compatibility with biological systems makes it a preferred choice for buffering in pharmaceutical formulations and vaccine development.