Phosphate Buffer (PB Buffer) is widely used in biological and chemical research due to its ability to maintain a stable pH environment. It is commonly employed in cell culture applications to provide optimal conditions for cell growth and viability. In molecular biology, PB Buffer is essential for DNA and RNA extraction, purification, and electrophoresis, ensuring the integrity of nucleic acids during experiments. It is also utilized in immunohistochemistry and immunofluorescence techniques to prepare tissue samples and maintain antibody stability. Additionally, PB Buffer plays a critical role in enzyme assays and protein studies, where consistent pH levels are crucial for accurate results. Its compatibility with various biochemical processes makes it a versatile tool in laboratories.