Phosphate Buffer (PB Buffer) is widely used in biological and chemical research due to its ability to maintain a stable pH. It is commonly employed in cell culture applications to provide a consistent environment for cell growth and experimentation. In molecular biology, PB Buffer is essential for procedures such as DNA and RNA extraction, where it helps stabilize nucleic acids during isolation. It is also utilized in immunohistochemistry and immunofluorescence techniques to wash and prepare tissue samples, ensuring optimal antibody binding and reducing background noise. Additionally, PB Buffer is a key component in enzyme assays and protein studies, where it maintains the pH necessary for enzymatic activity and protein stability. Its compatibility with various biological systems makes it a versatile tool in laboratory settings.