Phosphate Buffer is widely used in biological and chemical research due to its ability to maintain a stable pH environment. It is commonly employed in cell culture applications to provide optimal conditions for cell growth and maintenance. In molecular biology, it is utilized in various techniques such as DNA and RNA extraction, gel electrophoresis, and protein purification to ensure consistent pH levels. Additionally, it plays a critical role in immunoassays, including ELISA, by stabilizing the reaction environment. Phosphate Buffer is also used in diagnostic laboratories for preparing reagents and samples, ensuring accurate and reliable test results. Its buffering capacity makes it essential in pharmaceutical formulations to maintain the stability and efficacy of drugs.