Phosphate Buffer (PB Buffer) is widely used in biological and chemical research for maintaining stable pH levels in various experimental conditions. It is commonly employed in cell culture applications to provide an optimal environment for cell growth and viability. In molecular biology, PB Buffer is essential for procedures such as DNA and RNA extraction, electrophoresis, and protein purification, ensuring consistent pH during these processes. It is also utilized in immunohistochemistry and immunofluorescence techniques to wash and prepare tissue samples, preventing pH-induced degradation. Additionally, PB Buffer is a key component in enzyme assays and diagnostic kits, where precise pH control is critical for accurate results. Its buffering capacity makes it suitable for use in pharmaceutical formulations and vaccine development, where stability and compatibility are paramount.