Phosphatase Buffer-1 is widely used in biochemical and molecular biology research for the study of phosphatase enzymes. It provides an optimal pH environment for the activity of these enzymes, which are crucial in dephosphorylation processes. This buffer is essential in assays to determine phosphatase activity, often used in studying signal transduction pathways, cell cycle regulation, and metabolic processes. It is also utilized in protein research to remove phosphate groups from proteins, aiding in the analysis of phosphorylation states. Additionally, Phosphatase Buffer-1 is employed in diagnostic applications to detect enzyme activity in clinical samples, contributing to disease research and biomarker discovery.