Phosphate Buffered Saline with Tween 20 is widely used in biological and biochemical research. It serves as a versatile solution for washing cells and tissues, ensuring the maintenance of a stable pH and osmotic balance. The inclusion of Tween 20, a non-ionic surfactant, enhances its effectiveness by reducing surface tension, which aids in the even distribution of samples and prevents the nonspecific binding of proteins to surfaces. This makes it particularly valuable in immunoassays, such as ELISA, where it is used to wash microplate wells and minimize background noise. Additionally, it is employed in Western blotting to block membranes and wash away unbound antibodies, improving the specificity and clarity of the results. Its gentle yet effective properties also make it suitable for use in cell culture applications, where it helps to maintain cell integrity during experimental procedures.