Factor Xa Protease Digestion Buffer is specifically designed for use in the cleavage of fusion proteins that contain a Factor Xa protease recognition site. It is commonly employed in molecular biology and protein engineering workflows to isolate target proteins from fusion constructs. The buffer provides optimal conditions for Factor Xa protease activity, ensuring efficient and precise cleavage at the intended site. This is particularly useful in the purification of recombinant proteins, where the removal of affinity tags or fusion partners is necessary to obtain the functional protein of interest. Its application is critical in research involving protein expression, structural studies, and the production of biopharmaceuticals.