Nylon Membrane Stripping Buffer is primarily used in molecular biology and biochemistry laboratories for the removal of previously hybridized probes from nylon membranes. This process is essential when reprobing the same membrane with a different probe, allowing for multiple rounds of hybridization without the need for additional membranes. The buffer works by breaking the hydrogen bonds between the probe and the target DNA or RNA, effectively stripping the membrane clean. This application is particularly valuable in techniques such as Southern and Northern blotting, where it enhances efficiency and reduces costs by enabling the reuse of membranes. The buffer is designed to be gentle on the membrane while effectively removing the probe, ensuring the integrity of the membrane for subsequent use.