RNase-Free TE Buffer is widely used in molecular biology and genetic research for the preparation, dilution, and storage of nucleic acids, particularly RNA. Its primary application is to maintain the stability and integrity of RNA during experimental procedures, as it effectively chelates metal ions that could otherwise catalyze RNA degradation. This buffer is essential in techniques such as RNA extraction, reverse transcription, and PCR setup, where contamination by RNases must be avoided to ensure accurate and reliable results. Additionally, it is used in gel electrophoresis to resuspend RNA pellets and as a solvent for RNA samples prior to analysis. Its RNase-free property makes it a critical component in sensitive applications like RNA sequencing and gene expression studies.