This 0.5 M Phosphate Buffer (PB Buffer) at pH 9.0 is widely used in biological and chemical research due to its ability to maintain a stable pH in alkaline conditions. It is commonly employed in cell culture applications to provide a consistent environment for cell growth and viability. In molecular biology, PB Buffer is essential for procedures such as DNA and RNA extraction, where it helps stabilize nucleic acids during isolation. It is also utilized in immunohistochemistry and immunofluorescence techniques to wash and prepare tissue samples, ensuring minimal pH fluctuations that could affect staining results. Additionally, PB Buffer is a key component in enzyme assays, where it maintains optimal pH conditions for enzymatic activity. Its compatibility with various biochemical processes makes it a versatile tool in laboratory settings.