Universal KGB Buffer is widely used in molecular biology for various DNA manipulation techniques. It is particularly effective in DNA ligation reactions, providing optimal conditions for the activity of DNA ligase enzymes. The buffer is also utilized in restriction enzyme digestion, ensuring efficient and precise cutting of DNA at specific sites. Additionally, it is employed in PCR (polymerase chain reaction) setups to stabilize DNA polymerase and enhance the amplification process. Its compatibility with a range of enzymes and reactions makes it a versatile choice for researchers working on cloning, sequencing, and other genetic engineering applications.