Stauprimide is primarily utilized in biological research, particularly in the study of stem cell differentiation. It functions as a potent inhibitor of the c-Myc transcription factor pathway, which plays a critical role in maintaining the pluripotency of embryonic stem cells. By suppressing c-Myc activity, stauprimide enhances the efficiency of directed differentiation, allowing researchers to guide stem cells into specific cell lineages more effectively. This application is especially valuable in regenerative medicine and tissue engineering, where precise control over cell fate is essential. Additionally, stauprimide is used in cancer research to investigate the role of c-Myc in tumorigenesis and to explore potential therapeutic strategies targeting this pathway. Its ability to modulate cellular differentiation makes it a valuable tool in both developmental biology and oncology studies.