Used in biomedical research to quantify high molecular weight cytokeratins in human tissue samples and cell lysates. Helps in studying epithelial cell integrity and differentiation, particularly in cancer research where cytokeratin expression is a marker for tumor progression. Commonly applied in diagnosing and monitoring epithelial-derived cancers such as carcinomas of the lung, breast, and prostate. Also utilized in evaluating sample purity in cell culture and in biomarker validation studies for clinical diagnostics. Supports research in toxicology and drug development by assessing epithelial damage caused by therapeutic agents or environmental toxins.