Used in cancer research to quantify levels of the tumor suppressor protein p53 in cell lysates, tissue extracts, or biological fluids. Enables researchers to monitor p53 expression changes in response to DNA damage, oxidative stress, or therapeutic agents. Widely applied in studies of apoptosis, cell cycle regulation, and oncogenesis. Supports drug development by assessing the impact of novel compounds on p53 activation or stabilization. Provides a reliable, high-throughput method for biomarker validation in tumor models and preclinical studies.