FEN1-IN-1 is primarily used in biochemical research to study the role of flap endonuclease 1 (FEN1) in DNA repair and replication processes. It serves as a selective inhibitor of FEN1, enabling scientists to investigate its function in maintaining genomic stability. This compound is particularly valuable in cancer research, as it helps explore the potential of targeting FEN1 to disrupt DNA repair mechanisms in cancer cells, potentially leading to new therapeutic strategies. Additionally, FEN1-IN-1 is utilized in studies focused on understanding the molecular pathways involved in DNA damage response and cell cycle regulation. Its application extends to the development of assays to screen for compounds that modulate FEN1 activity, aiding in drug discovery efforts.