EHT 5372 is a potent and selective small-molecule inhibitor of the WDR5-MLL1 protein-protein interaction. It disrupts the MLL1 core complex, inhibiting histone H3 lysine 4 (H3K4) methylation essential for gene activation. Primarily used in biochemical research to study epigenetic mechanisms in mixed-lineage leukemia (MLL) and other cancers driven by MLL1 rearrangements. Valuable for investigating cell proliferation, survival pathways, drug discovery, in vitro assays, and in vivo models.