Wilsonine is primarily used in research settings for its biological activity, particularly in studies related to enzyme inhibition and cancer cell proliferation. It has shown potential as an antitumor agent due to its ability to interfere with specific signaling pathways in abnormal cells. Researchers also investigate its role in modulating immune responses and its possible application in developing therapies for inflammatory conditions. Due to its complex structure, it serves as a model compound in synthetic organic chemistry for developing new reaction methodologies.