Used in biomedical research to quantify levels of the retinoblastoma protein (pRB) in cell lysates or tissue samples. This kit enables researchers to study pRB expression and phosphorylation status, which are critical in understanding cell cycle regulation, tumor suppression mechanisms, and cancer progression. Commonly applied in oncology, molecular biology, and cellular signaling studies to assess the functional state of the pRB pathway in response to drugs, genetic modifications, or disease conditions. The ELISA format allows for high-throughput, sensitive, and specific detection, making it suitable for both basic research and preclinical investigation.