NESS-0327 is primarily utilized in research settings as a potent and selective cannabinoid receptor antagonist. It is particularly valuable in studies aimed at understanding the endocannabinoid system, as it helps to block CB1 receptors with high specificity. This makes it a useful tool for investigating the physiological and pathological roles of cannabinoid receptors in the brain and peripheral tissues. Researchers employ NESS-0327 to explore its potential in modulating appetite, pain perception, and neurological disorders. Its high affinity for CB1 receptors also makes it a candidate for studying addiction mechanisms and developing therapeutic strategies for substance abuse. Additionally, it is used in preclinical studies to dissect the effects of cannabinoids in various biological processes, providing insights into potential drug targets.