D-(-)-Cellobiose is widely used in biochemical research as a model compound to study cellulose degradation and enzymatic hydrolysis. It serves as a substrate for enzymes like cellulase, helping researchers understand the mechanisms of cellulose breakdown, which is crucial for biofuel production. In the food industry, it is utilized as a low-calorie sweetener and a prebiotic to promote gut health by stimulating the growth of beneficial bacteria. Additionally, it finds applications in the development of biodegradable materials and as a precursor in the synthesis of various bioactive compounds. Its role in studying glycosidic bonds also aids in advancing carbohydrate chemistry.