DL-Alanine-3-13C is widely used in metabolic studies and research involving isotope labeling. It serves as a tracer to investigate amino acid metabolism in biological systems, helping scientists understand pathways like protein synthesis and degradation. In nuclear magnetic resonance (NMR) spectroscopy, it acts as a labeled standard for structural and dynamic analysis of proteins and peptides. Additionally, it is employed in stable isotope probing to study microbial communities and their metabolic functions in environmental samples. Its application extends to medical research, where it aids in diagnosing metabolic disorders and monitoring therapeutic interventions.