Used as a stable isotope-labeled tracer in metabolic and physiological studies to monitor urea cycle activity and protein metabolism. Enables precise tracking of nitrogen and carbon flux in humans and animals without radiation exposure. Commonly employed in breath tests and mass spectrometry-based assays to diagnose gastrointestinal disorders such as H. pylori infection. Also utilized in agricultural research to study nitrogen utilization in plants and soil, improving fertilizer efficiency and reducing environmental impact.