Used primarily as a carbonic anhydrase inhibitor, it finds application in research settings to study fluid secretion and pH regulation in biological systems. It has been investigated for its potential to reduce intraocular pressure, making it relevant in ophthalmic research. Additionally, it is used to explore the role of carbonic anhydrase in tumor growth and cerebral edema. Its ability to cross cell membranes allows for effective intracellular enzyme inhibition, which is valuable in physiological and pharmacological studies involving ion transport and cellular acid-base balance.