JW 642 is primarily utilized in the field of medical research, particularly in the study of neurological disorders. It functions as a potent and selective inhibitor of the enzyme glutamate carboxypeptidase II (GCPII), which plays a significant role in modulating glutamate signaling in the brain. By inhibiting GCPII, JW 642 helps regulate excessive glutamate levels, which are often associated with conditions such as neuropathic pain, schizophrenia, and neurodegenerative diseases like Alzheimer's. Its application in preclinical studies provides valuable insights into the development of therapeutic strategies targeting glutamate-related pathologies. Additionally, JW 642 is used as a tool compound in biochemical assays to further understand the physiological and pathological roles of GCPII in the central nervous system.