CDK8-IN-3 is primarily used in research settings to study the role of cyclin-dependent kinase 8 (CDK8) in various biological processes. It is a selective inhibitor of CDK8, making it a valuable tool for investigating the kinase's involvement in transcriptional regulation, cell cycle control, and signaling pathways. Researchers utilize this compound to explore its potential effects on cancer cells, as CDK8 is often implicated in tumor growth and progression. By inhibiting CDK8, scientists can better understand its function in oncogenesis and evaluate its potential as a therapeutic target. Additionally, CDK8-IN-3 is used to study its impact on inflammatory responses and metabolic pathways, providing insights into broader disease mechanisms. Its application extends to drug discovery, where it serves as a lead compound for developing more potent and selective CDK8 inhibitors.