SU-5614 is primarily used in biochemical research as a potent inhibitor of the enzyme cyclin-dependent kinase 1 (CDK1). It plays a significant role in studying cell cycle regulation, particularly in understanding the mechanisms of mitosis and cell division. Researchers utilize SU-5614 to investigate the effects of CDK1 inhibition on cancer cell proliferation, as CDK1 is often overactive in various types of cancer. Additionally, it aids in exploring potential therapeutic strategies for targeting CDK1 in oncology. Its application extends to in vitro studies, where it helps elucidate the pathways involved in cell cycle control and apoptosis.