Cyclo(-RGDfK) is widely used in biomedical research due to its ability to specifically bind to integrin receptors, particularly αvβ3 integrin, which are overexpressed in tumor cells and endothelial cells of angiogenic blood vessels. This makes it a valuable tool in cancer research for targeting and imaging tumors, as well as in the development of anti-angiogenic therapies. It is also utilized in drug delivery systems to enhance the specificity and efficiency of therapeutic agents directed at cancer cells. Additionally, Cyclo(-RGDfK) is employed in tissue engineering to promote cell adhesion and migration, aiding in the regeneration of damaged tissues. Its role in studying cell signaling pathways related to integrin-mediated processes further underscores its importance in understanding various diseases.