Used primarily in cell biology research to manipulate and study intracellular calcium levels. It penetrates cell membranes easily and is hydrolyzed inside cells to release BAPTA, a potent calcium chelator. This allows researchers to buffer or reduce cytosolic calcium concentrations, helping to investigate calcium-dependent processes such as neurotransmitter release, muscle contraction, enzyme activity, and apoptosis. Frequently applied in live-cell imaging experiments to control calcium transients and assess their impact on cellular functions.