Thermolysin is widely used in the food industry for the production of aspartame, a low-calorie artificial sweetener. It catalyzes the condensation reaction between L-aspartic acid and L-phenylalanine methyl ester to form aspartame. In biochemistry and molecular biology, it is employed for peptide bond cleavage in protein sequencing and peptide synthesis due to its specificity for hydrophobic amino acids. Additionally, thermolysin is utilized in the preparation of bioactive peptides from proteins, which have applications in functional foods and nutraceuticals. Its stability at high temperatures makes it suitable for industrial processes requiring elevated temperatures.