Ajmalimine, also known as ajmaline, is an indole alkaloid derived from the roots of Rauwolfia species. It is primarily utilized in pharmaceutical and cardiovascular research as a class Ia antiarrhythmic agent. Ajmaline is widely employed in diagnostic testing for Brugada syndrome by intravenously challenging patients to unmask the condition through sodium channel blockade, which prolongs the QRS complex on ECG. It exhibits antihypertensive effects by influencing autonomic nervous system activity and is studied for its potential in treating cardiac arrhythmias and other cardiovascular disorders. Due to its specific mechanism of action on voltage-gated sodium channels and natural origin, Ajmalimine serves as a key compound in drug discovery for novel antiarrhythmic therapies with improved safety profiles.