HEPES
99%
- Product Code: 126966
Alias:
N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES); Hydroxyethylpiperazineethanesulfonic acid, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, N -(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid
CAS:
7365-45-9
| Molecular Weight: | 238.3 g./mol | Molecular Formula: | C₈H₁₈N₂O₄S |
|---|---|---|---|
| EC Number: | 230-907-9 | MDL Number: | MFCD00006158 |
| Melting Point: | 234-238 °C | Boiling Point: | |
| Density: | 1.07 g/mL at 20 °C | Storage Condition: | room temperature |
Product Description:
HEPES is widely used as a buffering agent in biological and biochemical research due to its ability to maintain a stable pH in cell cultures and enzymatic reactions. It is particularly effective in maintaining physiological pH levels (around 7.2–7.5) in cell culture media, making it ideal for growing mammalian cells. HEPES is also utilized in protein studies, as it minimizes interference with biochemical processes and does not form complexes with metal ions. Additionally, it is commonly employed in electrophoresis, chromatography, and other laboratory techniques where precise pH control is critical. Its low toxicity and compatibility with various biological systems make it a preferred choice in molecular biology and tissue culture applications.
Product Specification:
| Test | Specification |
|---|---|
| Purity (Titration With NaOH) (%) | 99-101 |
| UV Absorbance (1Mol/L, H2O, 250Nm) | 0-0.25 |
| UV Absorbance (1Mol/L, H2O, 260Nm) | 0-0.15 |
| UV Absorbance (1Mol/L, H2O, 320Nm) | 0-0.1 |
| Water By Karl Fischer (%) | 0-0.1 |
| Melting Point (Degree Celsius) | 234-238 |
| Appearance | White powder or crystals |
| Infrared Spectrum | Conforms to Structure |
| Solubility In Water | clear (17.5g/100mL) |
Sizes / Availability / Pricing:
| Size (g) | Availability | Price | Quantity |
|---|---|---|---|
| 25.000 | 10-20 days | ฿390.00 |
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| 100.000 | 10-20 days | ฿790.00 |
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| 500.000 | 10-20 days | ฿3,760.00 |
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| 1000.000 | 10-20 days | ฿7,500.00 |
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| 2500.000 | 10-20 days | ฿17,140.00 |
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HEPES
HEPES is widely used as a buffering agent in biological and biochemical research due to its ability to maintain a stable pH in cell cultures and enzymatic reactions. It is particularly effective in maintaining physiological pH levels (around 7.2–7.5) in cell culture media, making it ideal for growing mammalian cells. HEPES is also utilized in protein studies, as it minimizes interference with biochemical processes and does not form complexes with metal ions. Additionally, it is commonly employed in electrophoresis, chromatography, and other laboratory techniques where precise pH control is critical. Its low toxicity and compatibility with various biological systems make it a preferred choice in molecular biology and tissue culture applications.
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