HEPES
biotech grade
- Product Code: 126967
Alias:
N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES); Hydroxyethylpiperazineethanesulfonic acid, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, N -(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid
CAS:
7365-45-9
| Molecular Weight: | 238.3 g./mol | Molecular Formula: | C₈H₁₈N₂O₄S |
|---|---|---|---|
| EC Number: | 230-907-9 | MDL Number: | MFCD00006158 |
| Melting Point: | 234-238 °C | Boiling Point: | |
| Density: | 1.07 g/mL at 20 °C | Storage Condition: | room temperature |
Product Description:
HEPES is widely used as a buffering agent in biological and biochemical research due to its ability to maintain a stable pH in cell culture media and enzymatic reactions. It is particularly favored in cell and tissue culture applications because it is non-toxic to cells and does not interfere with biological processes. HEPES is also employed in protein studies, electrophoresis, and molecular biology experiments to ensure consistent pH levels, which is critical for maintaining protein stability and activity. Additionally, it is used in microscopy and imaging techniques to stabilize pH during live-cell imaging, ensuring accurate and reliable results. Its effectiveness in a wide pH range (6.8–8.2) makes it a versatile choice for various laboratory applications.
Product Specification:
| Test | Specification |
|---|---|
| As | 0ppm-0.1ppm |
| Ca | 0-10 |
| Co | 0ppm-5ppm |
| Cu | 0ppm-5ppm |
| Fe | 0ppm-5ppm |
| Ignition Residue (As Sulfate) | 0-0.001 |
| K | 0ppm-50ppm |
| Li | 0ppm-5ppm |
| Loss On Drying | 0-0.005 |
| Mg | 0-5 |
| Mo | 0ppm-5ppm |
| Na | 0-50 |
| Pb | 0-5 |
| Ph(25 Degree Celsius, 1 M In H2O) | 5-6.5 |
| Purity (T) | 0.995-1.005 |
| Sulfate (SO42-) | 0ppm-50ppm |
| Useful Ph Range | 6.8-8.2 |
| UV Absorption λ: 260 Nm A(0.5 M In H2O) | 0-0.1 |
| UV Absorption λ: 280 Nm A(0.5 M In H2O) | 0-0.1 |
| Insoluble Matter Passes Filter Test | PASSES FILTER TEST |
| Proteases | None Detected |
| Solubility H2O | Soluble1 M At 20 Degree Celsius, Clear, Colorless |
Sizes / Availability / Pricing:
| Size (g) | Availability | Price | Quantity |
|---|---|---|---|
| 5.000 | 10-20 days | Ft5,925.76 |
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| 25.000 | 10-20 days | Ft16,376.64 |
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| 100.000 | 10-20 days | Ft50,530.57 |
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| 500.000 | 10-20 days | Ft172,277.99 |
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HEPES
HEPES is widely used as a buffering agent in biological and biochemical research due to its ability to maintain a stable pH in cell culture media and enzymatic reactions. It is particularly favored in cell and tissue culture applications because it is non-toxic to cells and does not interfere with biological processes. HEPES is also employed in protein studies, electrophoresis, and molecular biology experiments to ensure consistent pH levels, which is critical for maintaining protein stability and activity. Additionally, it is used in microscopy and imaging techniques to stabilize pH during live-cell imaging, ensuring accurate and reliable results. Its effectiveness in a wide pH range (6.8–8.2) makes it a versatile choice for various laboratory applications.
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