Thiamine nitrate is a high-purity analytical standard (>99.6%) used in laboratories for calibration in chromatography (e.g., HPLC), spectroscopy, method validation, and quality control in analyzing vitamin B1 content in foods, pharmaceuticals, and biological samples.

It is widely used as a dietary supplement to prevent and treat vitamin B1 deficiency, which can lead to conditions like beriberi and Wernicke-Korsakoff syndrome. It is commonly added to fortified foods, such as cereals and bread, to enhance their nutritional value. In agriculture, it is used as a growth stimulant for plants, improving their overall health and resistance to stress. Additionally, it finds application in animal feed to ensure proper growth and prevent thiamine deficiency in livestock. In the pharmaceutical industry, it is a key ingredient in multivitamin formulations and therapeutic products aimed at supporting nervous system function and energy metabolism.