Methyltestosterone solution is primarily used in medical settings to address conditions related to low testosterone levels in males, such as delayed puberty, impotence, or hormonal imbalances. It helps in promoting the development of male sexual characteristics and improving muscle mass and strength.

In veterinary medicine, it is sometimes administered to animals to treat similar hormonal deficiencies or to enhance growth and performance.

Additionally, it has been explored in research for its potential effects on certain types of anemia, as it can stimulate red blood cell production. However, its use is strictly regulated due to potential side effects and misuse in athletic performance enhancement.