Used as a sensitive reagent for the detection and quantification of copper ions in various samples, including water, biological fluids, and industrial materials. It forms a stable, colored complex with copper, making it valuable in spectrophotometric analysis. Applied in environmental monitoring to assess copper contamination levels. Utilized in biochemistry and molecular biology research for studying copper-dependent enzymes and proteins. Also employed in chemical synthesis as a chelating agent to control copper-catalyzed reactions. Its stability and specificity make it a preferred choice in analytical and laboratory settings.