Used as a key intermediate in the synthesis of macrocyclic ligands, particularly in the preparation of functionalized tetraazacyclododecane (DO3A) derivatives. These derivatives are widely employed in the development of MRI contrast agents due to their ability to form stable complexes with lanthanide ions such as gadolinium(III). The tert-butyl protecting groups allow selective modification of the nitrogen atoms, enabling controlled conjugation to targeting molecules or biomolecules in diagnostic imaging applications. Also utilized in radiopharmaceuticals and chelation therapy research owing to its favorable coordination properties and stability under physiological conditions.