Cupferron solution is widely used as a chelating agent in analytical chemistry, particularly for the separation and determination of metal ions. It forms stable, water-insoluble complexes with a variety of metals such as iron, copper, aluminum, and rare earth elements, allowing their extraction from aqueous solutions into organic solvents like ether or chloroform. This property makes it valuable in gravimetric and spectrophotometric analyses. It is commonly employed in the removal of interfering metal ions during trace metal analysis, enhancing the accuracy of results. Cupferron is also used in the purification of metal salts and in the preparation of samples for instrumental analysis, such as atomic absorption or ICP-MS, by masking or eliminating certain metal interferences. Due to its sensitivity and selectivity toward specific metals, it plays a role in environmental testing, metallurgical analysis, and quality control in industrial processes. However, because cupferron is unstable in storage and decomposes over time, it is typically prepared fresh and used immediately to ensure optimal performance.