Widely used as a chelating agent in medical and analytical applications, this compound effectively binds multivalent metal ions such as calcium, magnesium, and transition metals. It is particularly valuable in biochemical assays where control of metal ion activity is critical, such as in enzyme inhibition studies or stabilization of nucleic acids. Due to its multiple binding sites and structural stability, it enhances the selectivity and efficiency of metal ion sequestration in complex biological matrices. It also finds use in diagnostic reagents and as a component in buffers for molecular biology techniques, including PCR and electrophoresis, where precise metal ion regulation improves reaction fidelity.