Cupferron is widely used as a chelating agent in analytical chemistry for the separation and determination of metal ions. It forms stable complexes with metals like iron, copper, and vanadium, allowing for their selective precipitation from solutions. This property makes it valuable in qualitative and quantitative analysis, particularly in isolating specific metals from complex mixtures. Additionally, Cupferron is employed in the purification of metal ores and in the synthesis of organometallic compounds. Its ability to selectively bind to certain metals also finds applications in environmental testing, where it helps detect and measure trace metal contaminants in water and soil samples.