N-Acetyl-DL-penicillamine is primarily used in the field of medical research and treatment, particularly in the study and management of heavy metal poisoning. It acts as a chelating agent, binding to toxic metals such as mercury, lead, and copper, facilitating their excretion from the body. This property makes it valuable in detoxification therapies, where it helps reduce the harmful effects of metal accumulation in tissues. Additionally, it is investigated for its potential role in treating certain autoimmune disorders and inflammatory conditions due to its ability to modulate immune responses. Its application extends to biochemical research, where it is used to study enzyme inhibition and protein interactions.