Tri-tert-butyl 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate is primarily used as a precursor in the synthesis of macrocyclic ligands, particularly in the development of chelating agents. These ligands are essential in coordination chemistry, where they form stable complexes with metal ions. Such complexes are widely applied in medical imaging, especially in magnetic resonance imaging (MRI) as contrast agents, enhancing the visibility of internal body structures. Additionally, this compound plays a role in catalysis, where it helps in the stabilization of metal catalysts used in various organic transformations. Its ability to bind selectively to specific metals also makes it valuable in environmental chemistry for metal ion extraction and separation processes.