Used in chemiluminescence imaging for detecting reactive oxygen species (ROS) in biological systems. It helps study oxidative stress in cells and tissues, aiding research in diseases like cancer and neurodegeneration. Applied in vitro and in vivo to monitor ROS levels with high sensitivity. Also utilized in drug development to assess the efficacy of antioxidants and ROS-scavenging compounds. Its application extends to environmental studies for evaluating oxidative damage in organisms exposed to pollutants.