Used primarily in forensic science to detect trace amounts of blood at crime scenes. When mixed with an oxidizing agent and exposed to hemoglobin, it produces a bright blue chemiluminescent glow, allowing visualization of blood even if cleaned or diluted. This reaction is highly sensitive and effective in low-light conditions, making it ideal for crime scene investigations. Also employed in biochemical research to detect reactive oxygen species and in immunoassays as a label for detecting specific proteins. Its ability to emit light upon oxidation makes it valuable in analytical techniques requiring high sensitivity.