DL-tert-Leucine is widely used in the pharmaceutical industry as a chiral building block for the synthesis of various drugs. Its unique structure makes it valuable in creating active pharmaceutical ingredients (APIs) with enhanced stability and bioavailability. It is particularly important in the development of protease inhibitors, which are used to treat diseases such as HIV and hepatitis C. Additionally, DL-tert-Leucine serves as a key intermediate in the production of peptidomimetics, which mimic the function of natural peptides and are used in drug design to target specific biological pathways. Its application extends to the field of agrochemicals, where it is utilized in the synthesis of herbicides and fungicides. The compound’s steric hindrance also makes it useful in asymmetric synthesis, enabling the production of enantiomerically pure compounds.