L-Norvaline Ethyl Ester Hydrochloride is primarily used in biochemical research and pharmaceutical development. It serves as a building block in the synthesis of peptides and amino acid derivatives, which are essential for studying protein structure and function. This compound is also utilized in the preparation of enzyme inhibitors, aiding in the exploration of enzymatic pathways and potential therapeutic targets. Additionally, it finds application in the development of prodrugs, where it helps enhance the bioavailability and stability of active pharmaceutical ingredients. Its role in organic synthesis further extends to creating chiral intermediates for the production of complex molecules in medicinal chemistry.