Cholesteryl behenate is widely used in the pharmaceutical and cosmetic industries due to its unique properties. In pharmaceuticals, it serves as an excipient in drug formulations, particularly in controlled-release systems. Its ability to form stable lipid bilayers makes it suitable for use in liposomes, which are employed for targeted drug delivery. This helps in enhancing the bioavailability of poorly soluble drugs and reduces side effects by directing the drug to specific tissues or cells.

In cosmetics, it acts as an emulsifier and stabilizer in creams and lotions. Its lipid-like structure helps in maintaining the integrity of the product, ensuring a smooth texture and prolonged shelf life. Additionally, it is used in skincare products for its moisturizing properties, helping to improve skin hydration and elasticity.

Cholesteryl behenate is also utilized in research laboratories as a standard in chromatography for lipid analysis. Its well-defined properties make it a reliable reference material for studying lipid behavior and interactions in various biological systems.