Column-layer chromatographic silica gel is widely used in the separation and purification of complex mixtures in both analytical and preparative chemistry. It serves as a stationary phase in chromatography, enabling the separation of compounds based on their polarity and molecular size. In column chromatography, it is packed into a column to isolate and purify substances such as natural products, pharmaceuticals, and organic compounds. In thin-layer chromatography (TLC), it is coated onto plates to analyze and identify components in a mixture quickly. Its high surface area and porosity make it effective for adsorbing and separating a wide range of compounds. Additionally, it is utilized in the purification of proteins, peptides, and other biomolecules in biochemical research. Its versatility and efficiency make it a critical tool in laboratories for quality control, research, and industrial applications.