Caspase-1/4 Chromogenic Substrate is widely used in biochemical research to study the activity of caspase-1 and caspase-4 enzymes. These enzymes play a critical role in inflammatory processes and programmed cell death (apoptosis). The substrate is specifically designed to produce a detectable color change upon cleavage by the caspases, allowing researchers to quantify enzyme activity in vitro. This tool is particularly valuable in studying the mechanisms of inflammation, immune responses, and diseases such as sepsis, neurodegenerative disorders, and cancer. It is also utilized in drug discovery to screen and evaluate potential inhibitors of caspase-1 and caspase-4, aiding in the development of therapeutic agents targeting inflammatory and apoptotic pathways.