Used as a staining agent in histology and cytology to detect specific enzymes, particularly in the visualization of alkaline phosphatase activity. Applied in biochemical assays for identifying and studying enzyme distribution in tissues. Employed in the development of diagnostic kits for medical and research purposes. Utilized in forensic science for detecting biological materials in crime scene investigations. Acts as a chromogenic substrate in various enzymatic reactions, aiding in the analysis of enzyme kinetics. Commonly used in laboratory settings for its ability to produce a distinct color change, facilitating easy interpretation of results. Also finds application in the textile industry as a dye intermediate for specific fabric treatments.